免疫荧光
化学
细胞毒性
共焦
缺氧(环境)
标签
荧光
单克隆抗体
分子生物学
加合物
衍生工具(金融)
共焦显微镜
生物化学
抗体
生物
氧气
细胞生物学
免疫学
体外
物理
几何学
数学
有机化学
量子力学
经济
金融经济学
作者
Jeffrey B. Matthews,Hans Adomat,Nicholas Farrell,Patrick J. King,Cameron J. Koch,Edith M. Lord,Branko Palcic,Neal Poulin,J. Sangulin,Kirsten A. Skov
出处
期刊:PubMed
日期:1996-07-01
卷期号:27: S200-3
摘要
The monoclonal antibody ELK3-51 was previously developed to detect adducts of the 2-nitroimidazole EF5. Direct immunofluorescence was used to detect adducts of EF5 or of a platinated derivative cis-[PtCl2(NH3)EF5] in SCCVII cells treated under aerobic or hypoxic conditions. Fluorescence measurements of these cells using both image and flow cytometric methods were compared, giving similar profiles. Platination significantly decreased immunofluorescence levels (approximately 4-fold less than EF5) after 3 h in hypoxia, but also increased levels after exposure in air (approximately 1.5 x) such that the hypoxic ratio decreased from approximately 50 to approximately 13. Platinated EF5 also showed significantly greater cytotoxicity than its parent in both aerobic and hypoxic cells. These results are consistent with targeting of EF5 to DNA, which was confirmed qualitatively by confocal microscopy.
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