生物膜
粘质沙雷氏菌
微生物学
群体感应
细菌
氧氟沙星
化学
生物
抗生素
生物化学
基因
大肠杆菌
环丙沙星
遗传学
作者
Huai-Zhi Luo,Jinwei Zhou,Bing Sun,Huan Jiang,Shi Tang,Ai‐Qun Jia
出处
期刊:Biofouling
[Informa]
日期:2021-02-07
卷期号:37 (2): 145-160
被引量:8
标识
DOI:10.1080/08927014.2021.1874942
摘要
Serratia marcescens NJ01, a Gram-negative bacterium, can infect tomato leaves and cause chlorosis and wilting. The present study evaluated the quorum sensing (QS) and biofilm inhibitory effects of seven carboline compounds against S. marcescens NJ01 at 20 μg ml−1, and subsequently focused the study on norharmane as this had the best inhibitory activity. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis confirmed the down-regulation of QS and biofilm related genes bsmA, bsmB, fimA, fimC, flhD, pigA, pigC and shlA on exposure to norharmane. Fourier-Transform Infrared Spectroscopy (FT-IR) analysis showed a reduction in the major components of the exopolysaccharide (EPS) matrix such as nucleic acids, proteins and fatty acids, which are involved in forming the tertiary structure of biofilms. Norharmane exposure also enhanced the susceptibility of the biofilm to ofloxacin. Hence, norharmane has the potential for use as an antibiotic adjuvant to enhance the efficacy of conventional antibiotics to reduce pathogenic bacterial infections.
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