生物
胚胎干细胞
转录组
基因表达
拟南芥
表观遗传学
遗传学
基因
细胞生物学
基因组
基因表达调控
拟南芥
计算生物学
突变体
作者
Ping Kao,Michael A. Schon,Magdalena Mosiołek,Balaji Enugutti,Michael D. Nodine
出处
期刊:Development
[The Company of Biologists]
日期:2021-07-06
卷期号:148 (13)
被引量:24
摘要
ABSTRACT Soon after fertilization of egg and sperm, plant genomes become transcriptionally activated and drive a series of coordinated cell divisions to form the basic body plan during embryogenesis. Early embryonic cells rapidly diversify from each other, and investigation of the corresponding gene expression dynamics can help elucidate underlying cellular differentiation programs. However, current plant embryonic transcriptome datasets either lack cell-specific information or have RNA contamination from surrounding non-embryonic tissues. We have coupled fluorescence-activated nuclei sorting together with single-nucleus mRNA-sequencing to construct a gene expression atlas of Arabidopsis thaliana early embryos at single-cell resolution. In addition to characterizing cell-specific transcriptomes, we found evidence that distinct epigenetic and transcriptional regulatory mechanisms operate across emerging embryonic cell types. These datasets and analyses, as well as the approach we devised, are expected to facilitate the discovery of molecular mechanisms underlying pattern formation in plant embryos. This article has an associated ‘The people behind the papers’ interview.
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