Engineered Metal-Binding Proteins: Purification to Protein Folding

Proteins can make use of metal ions to bind substrates, \nto maintain structure, to effect catalysis, and for \nallosteric control and regulation. In order to hold a particular \nmetal ion with high affinity and specificity, proteins form multidentate \nbinding pockets designed to fulfill both the chemical and \ngeometric bonding requirements of that metal. Metal recognition \ncan be engineered into proteins for applications such as protein \npurification.