唾液酸
糖蛋白
化学
生物素
生物化学
聚糖
肟
细胞
N-乙酰神经氨酸
醛
苯胺
催化作用
有机化学
作者
Ying Zeng,T.N.C. Ramya,Anouk Dirksen,Philip E. Dawson,James C. Paulson
出处
期刊:Nature Methods
[Springer Nature]
日期:2009-02-22
卷期号:6 (3): 207-209
被引量:368
摘要
We describe a simple method for efficiently labeling cell-surface sialic acid-containing glycans on living animal cells. The method uses mild periodate oxidation to generate an aldehyde on sialic acids, followed by aniline-catalyzed oxime ligation with a suitable tag. Aniline catalysis dramatically accelerates oxime ligation, allowing use of low concentrations of aminooxy-biotin at neutral pH to label the majority of cell-surface sialylated glycoproteins while maintaining high cell viability.
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