Stable Isotope Tracing of Water Sources and Recharge Contributions to Qinghai Lake, Northeastern Qinghai–Tibet Plateau

To elucidate the recharge relationships and hydrological processes among different water bodies in the Qinghai Lake Basin, precipitation, river water, groundwater, and lake water samples were collected from April to November 2024. The hydrogen and oxygen stable isotope compositions (δ2H and δ18O) were determined to characterize their spatiotemporal variations, and the MixSIAR model was applied to quantitatively evaluate the recharge contributions among different water bodies. The results show significant differences in stable isotope compositions among the various water bodies. Overall, lake water exhibited the most enriched isotopic signatures, whereas groundwater was the most depleted and isotopically stable, while precipitation displayed the largest variability. Precipitation isotopes exhibited pronounced seasonal effects. River water showed a depletion–enrichment–depletion pattern, reflecting the important recharge contributions from wet season precipitation and frozen-soil meltwater. Groundwater exhibited relatively weak seasonal variations and a distinct smoothing effect. In contrast, the isotopic composition of lake water was jointly controlled by evaporative fractionation and multiple recharge sources, with the highest enrichment occurring in spring and gradual depletion during wet season and dry season. Spatially, significant isotopic differences were observed among rivers. Groundwater was relatively enriched in the western part of the basin and depleted in the eastern part, whereas lake water exhibited an opposite pattern, characterized by enrichment in the east and depletion in the west. The Local Meteoric Water Line (LMWL) of the Qinghai Lake Basin was defined as δ2H = 8.07δ18O + 37.48 (R2 = 0.96). Both the slope and intercept were higher than those of the Global Meteoric Water Line (GMWL), indicating that locally recycled evaporated moisture played an important role in regional precipitation formation. The river water line showed characteristics similar to those of the groundwater line, suggesting strong hydraulic connectivity between river water and groundwater. In contrast, the lake water line deviated markedly from the meteoric water line, indicating significant evaporative fractionation of lake water. The MixSIAR results indicate that the contributions of river water, groundwater, and precipitation to lake water were 37.5%, 33.9%, and 28.6%, respectively. These findings reveal the complex hydrological connections and transformation processes among multiple water bodies in the Qinghai Lake Basin and provide a scientific basis for water resource management and ecological environmental protection in inland basins of the Qinghai–Tibet Plateau.