The assessment of the amplified efficacy of single cell PCR by diffent lysis buffers.

Objective: To assess the amplified efficacy of singlecell PCR by diffent lysis buffers and choose the better lysis method. Methods: Single lymphocyte was isolated under the inverted microscope equipped with micromanipulations. Two sorts of cell lysis methods (freezing-thawing and proteinase K lysis) were compared by their efficacy of multiplex amplification of DXZ1 and DYZ1 from a single cell. Results: Under the inverted microscope equipped with micromanipulations, 80 lymphocytes were isolated. The amplification efficacy was 100% with cell lysis of proteinase K, and 80% with cell lysis of freezing-thawing. The amplification efficacy of two cell lysis methods showed significant difference (χ2=6.81,P0.01). Conclusions:Proteinase K cell lysis method is simple and rapid. It can result in high amplification efficacy and meet to cell lysis and preparation of DNA template for amplification.