Clone expression and identification of the gene SLT-IIeA in E. coli

The SLT-IIeA gene was cut off from the recombined pSLT-IIeA which contained the SLT-IIeA gene and transferred into the plasmid vector pGEX-6p-1. The gene was genetically inserted at the downstream of the 3'terminus of the gene coding for enzyme glutathione S-transferase, which served as a carrier in this expression system. Recombinant expression plasmid ppSLT-IIeA was constructed, and transferred into E.coli BL21.After induced with IPTG, FedA was expressed as fusion protein linked with the GST. The fusion protein was designated as GST-SLT-IIeA. The res μLts of SDS-PAGE and Western-blot assay demonstrated that the protein expressed by the recombinant plasmid ppSLT-IIeA can specifically response to monoclonal antibody against SLT-IIeA.This indicated that the expected protein was successf μLly obtained.The above reserch is the first report on expression of SLT-IIeA of ED, and will lay foundation to the prevention of ED by gene engineer vaccines.