The study of the blood-brain barrier-perviousness of a bovine hydrolyzed peptides of casein

Objective To study the blood-brain barrier perviousness of the hydrolyzed peptides of casein which derived from milk.Methods A type of hychrolyzed peptide was injected into rat carotid vein and detected its concentration in serum and cerebrospinal fluid(CSF) at different time spot.The peptide sample was analyzed by HPLC after pretreatment.Results The sample peptides were very easy to be metabolized in systemic circulation and they were undetectable after intravenous administration for 6 minutes,and the peak intensity of the peptides decreased as time goes by within 6 minutes.Meanwhile two new peaks appeared in the CSF sample at 3 and 4 minutes spot.The retention time of those new peaks were very close to one of the peaks of peptide sample.Conclusion This result suggest that some peptides in the bovine hydrolyzed peptides of casein could cross the blood-brain barrier or the concentration of some molecules in the CSF have been changed.Meanwhile food-origin natural peptides may be possible to cross the BBB and pass into the CNS to change the function or even the structure of central nervous system.