The P.stutzeri strain named as WIT-1 with the ability of heterotrophic nitrification and aerobic denitrification was studied for its characteristics of nitrogen removal.The maximum tolerance of removing nitrate,nitrate removing and its capability of removing ammonia for non-sterilized sanitary sewage of WIT-1 were investigated.The results showed that initiating chemical oxygen demand(COD) concentration was 500 mg/L,the removal rate of nitrate was 96.38% in 48 h.At the same condition of COD,the strain WIT-1 maximum tolerance of removed nitrate concentration was 800 mg/L.When the amount of inoculation of the strain WIT-1 was 2%,the removal rate of ammonia was 32.741% in 12 h.When the initial COD concentration was 500 mg/L or the inoculation was improved to 8%,the removal rate of ammonia was 52.765% and 100% in 12 h,respectively.However,the removal rate of ammonia was only about 20% in 48 h.