Optimization of Agrobacterium-mediated Transformation Method for Rice

[Objective] This study aimed to optimize the method for Agrobacterium-mediated transformation in rice.[Method] Transgenic rice seedlings were obtained by optimizing and modifying the classical transformation methods.[Result] Mature embryos were adopted and sterilized by HgCl2.Transformation efficiency had achieved the maximum when OD600 was 0.10,which had increased by 23%,comparing with previous method.[Conclusion] The study provided a technical reference for relative researches of Agrobacterium-mediated transformation in rice.