Objective Classified HBV DNA of AsC of children living in Guangzhou. Methods Using a genotyping method based on the restriction fragment length polymorphism (RFLP) of amplified segment of the HBV S region. Results Among the AsC of children living in Guangzhou ,genotype B is 62.7%,genotype C is 33.3% genotype B and C is 3.3%,only 1.7%couldn not be classified . Conclusions The method for genotyping is simple and convenient,The prevalent HBV strain of children in Guangzhou is genotype B and genotype C.