Effect of raw/stewed Rhubarb in Xiayuxue Decoction on vascular endothelial function and microcirculation of rats with heat accumulation of blood stasis

Objective To study the effect of raw/stewed Rhubarb in Xiayuxue Decoction on vascular endothelial function and microcirculation of rats with heat accumulation of blood stasis Methods Seventy SD rats were divided into seven groups. The rats in experimental groups were given heat drugs of traditional Chinese medicine for 28 d, then injected adrenaline hydrochloride for later 7 d, twice a day. At the same time, those experimental rats were given different doses of Xiayuxue Decoction(9 and 4.5 g·kg-1) orally for 7 d. Then blood rheologic parameters such as ET, NO, PGI2, and VWF in rats were detected. Results Xiayuxue Decoction A/B have improved vascular endothelial cell injury and the microcirculation of the rats with heat accumulation of blood stasis. Compared with Xiayuxue Decoction A, each dose of Xiayuxue Decoction B has improved the levels of ET, NO, PGI2, and vWF factor significantly(P 0.05, 0.01). The efficiency on promoting blood circulation to remove blood stasis of Xiayuxue Decoction B was definitely better than Xiayuxue Decoction A. Conclusion The mechanism of promoting blood circulation to remove blood stasis may be through regulating the levels of ET, NO, PGI2, and VWF factor.