Chemical constituents from the leaves of Camellia sinensis

Objective To isolate the chemical constituents and elucidate their structures from the leaves of Camellia sinensis.Methods The compounds were isolated by chromatography on silica gel,Sephadex LH-20 column and recrystallizing methods.NMR and MS were employed for the structure identification.Results Eight compounds were obtained and elucidated as:caffeine(1),theobromine(2),kaempferol(3),(+)-catechin(4),daucosterol(5),(Z)-9-hexadecenoic acid(6),phenyl-β-naphthylamine(7),gallic acid(8),respectively.Conclusions Among these compounds,5,6 and 7 are obtained from the Camellia genus for the first time.