Expression and its clinical significance of tumor necrosis factor-α-induced protein-8 like-3 in colonic mucosa of patients with colon cancer

Objective To detect the expression of tumor necrosis factor-α-induced protein-8 like-3 (TIPE3) in colonic mucosa of patients with colon cancer, and to analyze the correlation between its abnormal expression and clinicopathological features of patients with colon cancer. Methods The expression of TIPE3 mRNA in 58 cases of colon cancer and tumor-adjacent tissues was detected by real-time polymerase chain reaction (RT-PCR). The expression of TIPE3 at protein level in 83 cases of colon cancer and tumor-adjacent tissues was determined by SP immunohistochemistry. Nonparametric rank-sum test and chi-square test were performed for statistical analysis. Results The relative expression of TIPE3 mRNA in the colon cancer tissues was 0.719 (0.104 to 0.887), which was lower than that of tumor-adjacent tissues (4.770, 1.732 to 6.800), and the difference was statistically significant (Z=-6.345, P 0.05). The expression of TIPE3 mRNA in group of patients with lymph node metastasis (0.113, 0.061 to 0.375) was lower than group of patients without lymph node metastasis (0.489, 0.327 to 0.956; Z=-3.815, P 0.05). The positive expression rate of patients at stage Ⅱ was higher than that of patients at stage Ⅲ (60.5%, 23/38 vs 29.7%, 1/37); and the difference was statistically significant (χ2=7.174, P<0.05). The positive expression rate of TIPE3 in group of patients with lymph node metastasis was lower than that of groups of patients without lymph node metastasis (28.2%, 11/39 vs 59.1%, 26/44), and the difference was statistically significant (χ2=7.983, P=0.005). Conclusions The expression of TIPE3 in colon cancer tissues is lower than that in tumor-adjacent tissues. Furthermore, it is correlated with lymph node metastasis, recurrence rate and survival rate. TIPE3 may be involved in the genesis, development, invasion and metastasis of colon cancer. Key words: Colonic neoplasms; Neoplasm metastasis; TIPE3