In vitro micropropagation of gerbera using auxiliary bud.

A protocol for rapid clonal micropropagation of gerbera was developed by auxillary bud.MS+BAP 2.0+NAA 0.5 mg/l, which was given early bud initiation (19.40 days), with higher initiation per cent (91.66%) along with longer shoot length (2.5 cm). MS+BAP 2.0+NAA 0.5+Ads 100 mg/ l produced higher number of shoots (7.33 shoots/explants). The micro shoots were rooted (96%) in just 8.56 days on MS+NAA 2.0+activated charcoal 750 mg/l. In vitro rooted plants were acclimatized on sand + soil + FYM+ leaf mould. Plants shows 82.43 per cent survival rate during acclimatization. The plants were established in the field after acclimatization.