纤毛
活体细胞成像
细胞器
细胞生物学
运动纤毛
细胞
细胞培养
小学(天文学)
生物
化学
物理
遗传学
天文
作者
Lucia Binó,Erika Mikulenková,Luděk Štěpánek,Ondřej Bernatík,David Vysloužil,Petra Pejšková,Peter Gorilák,Martina Huranová,Vladimír Varga,Lukáš Čajánek
出处
期刊:STAR protocols
[Elsevier]
日期:2022-03-01
卷期号:3 (1): 101199-101199
被引量:2
标识
DOI:10.1016/j.xpro.2022.101199
摘要
Primary cilia are hair-like sensory organelles protruding from the surface of most human cells. As cilia are dynamic, several aspects of their biology can only be revealed by real-time analysis in living cells. Here we describe the generation of primary cilia reporter cell lines. Furthermore, we provide a detailed protocol of how to use the reporter cell lines for live-cell imaging microscopy analysis of primary cilia to study their growth as well as intraciliary transport. For complete details on the use and execution of this protocol, please refer to Bernatik et al. (2020) and Pejskova et al. (2020).
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