胚胎干细胞
同源盒蛋白纳米
干细胞
转录因子
细胞效价
SOX2
染色质
表观遗传学
KLF4公司
作者
Yang Zhao,Ting Zhao,Jingyang Guan,Xu Zhang,Yao Fu,Junqing Ye,Jialiang Zhu,Gaofan Meng,Jian Ge,Susu Yang,Lin Cheng,Yaqin Du,Chaoran Zhao,Ting Wang,Linlin Su,Weifeng Yang,Hongkui Deng
出处
期刊:Cell
[Elsevier]
日期:2015-12-17
卷期号:163 (7): 1678-1691
被引量:142
标识
DOI:10.1016/j.cell.2015.11.017
摘要
Somatic cells can be reprogrammed into pluripotent stem cells (PSCs) by using pure chemicals, providing a different paradigm to study somatic reprogramming. However, the cell fate dynamics and molecular events that occur during the chemical reprogramming process remain unclear. We now show that the chemical reprogramming process requires the early formation of extra-embryonic endoderm (XEN)-like cells and a late transition from XEN-like cells to chemically-induced (Ci)PSCs, a unique route that fundamentally differs from the pathway of transcription factor-induced reprogramming. Moreover, precise manipulation of the cell fate transition in a step-wise manner through the XEN-like state allows us to identify small-molecule boosters and establish a robust chemical reprogramming system with a yield up to 1,000-fold greater than that of the previously reported protocol. These findings demonstrate that chemical reprogramming is a promising approach to manipulate cell fates.
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