粘附
材料科学
自愈水凝胶
细胞粘附
血小板
纳米技术
蛋白质吸附
血小板粘附
微流控
聚苯乙烯
生物物理学
生物医学工程
聚合物
高分子化学
复合材料
血小板聚集
免疫学
生物
医学
作者
Tobias Ekblad,Lars Faxälv,Olof Andersson,Nanny Wallmark,Andréas Larsson,Tomas Lindahl,Bo Liedberg
标识
DOI:10.1002/adfm.201000083
摘要
Abstract This work describes the preparation and properties of hydrogel surface chemistries enabling controlled and well‐defined cell adhesion. The hydrogels may be prepared directly on plastic substrates, such as polystyrene slides or dishes, using a quick and experimentally simple photopolymerization process, compatible with photolithographic and microfluidic patterning methods. The intended application for these materials is as substrates for diagnostic cell adhesion assays, particularly for the analysis of human platelet function. The non‐specific adsorption of fibrinogen, a platelet adhesion promoting protein, is shown to be completely inhibited by the hydrogel, provided that the film thickness is sufficient (>5 nm). This allows the hydrogel to be used as a matrix for presenting selected bioactive ligands without risking interference from non‐specifically adsorbed platelet adhesion factors, even in undiluted whole blood and blood plasma. This concept is demonstrated by preparing patterns of proteins on hydrogel surfaces, resulting in highly controlled platelet adhesion. Further insights into the protein immobilization and platelet adhesion processes are provided by studies using imaging surface plasmon resonance. The hydrogel surfaces used in this work appear to provide an ideal platform for cell adhesion studies of platelets, and potentially also for other cell types.
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