A Simple Procedure for Isolation, Culture of Protoplast, and Plant Regeneration

This chapter presents different steps involved in isolation and culture of protoplast; discusses basic steps involved in the developmental sequence from protoplast to plant; and overviews a simple procedure for protoplast isolation, culture, and plant regeneration. Protoplasts are naked plant cells without the cell wall, but they possess plasma membrane and other components of cell. Protoplasts are potentially totipotent individuals at the single-cell level. They have the potential to regenerate a cell wall, dedifferentiate, divide mitotically and form unlimited growing cell clones, and differentiate shoot and root meristems or embryos which regenerate whole plant.