Clerodane Diterpenoid Glucosides from the Stems of Tinospora sinensis

Ten new clerodane diterpenoid glucosides ( 1 – 10 ) and three known analogues ( 11 – 13 ) were isolated from an EtOAc extract of the stems of Tinospora sinensis . Spectroscopic analyses and chemical methods were used to elucidate the structures of these isolates. The absolute configurations of tinosinenosides A–C ( 1 – 3 ) were established by using experimental and calculated ECD data. Their cytotoxicity against the human epithelioid cervical carcinoma (HeLa) cell line and the nitric oxide production inhibitory activity of lipopolysaccharide-activated N9 microglial cells were tested. 1-Deacetyltinosposide A ( 12 ) exhibited mild cytotoxicity against HeLa cells, with an IC 50 value of 8.35 ± 0.60 μM.