First Report of Hippeastrum mosaic virus in Hippeastrum spp. in Mainland China

HomePlant DiseaseVol. 101, No. 6First Report of Hippeastrum mosaic virus in Hippeastrum spp. in Mainland China PreviousNext DISEASE NOTES OPENOpen Access licenseFirst Report of Hippeastrum mosaic virus in Hippeastrum spp. in Mainland ChinaX. H. Xu, W. Tang, R. Gao, S. K. Yang, F. Li, H. W. Sun, and X. B. LuX. H. XuSearch for more papers by this author, W. TangSearch for more papers by this author, R. GaoSearch for more papers by this author, S. K. YangSearch for more papers by this author, F. LiSearch for more papers by this author, H. W. SunSearch for more papers by this author, and X. B. LuSearch for more papers by this authorAffiliationsAuthors and Affiliations X. H. Xu W. Tang R. Gao S. K. Yang F. Li H. W. Sun X. B. Lu , Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Shandong Key Laboratory of Plant Pathology, Ji’nan 250100, China. Published Online:4 Apr 2017https://doi.org/10.1094/PDIS-08-16-1109-PDNAboutSectionsSupplemental ToolsAdd to favoritesDownload CitationsTrack Citations ShareShare onFacebookTwitterLinked InRedditEmailWechat Potyvirus is the largest and most agriculturally important genus of plant viruses in the world (Jordan and Hammond 1991). Hippeastrum spp. is a genus of perennial, herbaceous, bulbous plants in mainland China, which usually has high ornamental value. From July to October in 2015, we collected eight samples of Hippeastrum spp. with mosaic symptoms of irregular dark and light green leaves from public gardens, private courtyards, and flower markets in Ji’nan, Shandong Province. Total RNAs were extracted from one nonsymptomatic and eight symptomatic samples with the RNAprep Pure Kit (for polysaccharides and polyphenolics-rich plant) (Tiangen, Beijing, China). cDNA was synthesized by reverse transcription using the M4T primer (5′-GGTCGACTGCAGGATCCAAGCT15-3′) (Chen et al. 2001) and a random primer. PCR was then performed with degenerate primers specific for potyviruses (NIb-F, 5′-GGNAAYAAYAGYGGNCARCC-3′ and M4T) (Chen et al. 2001), potexviruses (Potex 2RC, 5′-AGCATRGCNSCRTCYTG-3′ and Potex 5′-CAYCARCARGCMAARGAYGA-3′) (van der Vlugt and Berendsen 2002), and tobamoviruses (Tom-F, 5′-GTYGTTGATGAGTTCRTGGA-3′ and Tom-R, 5′-ATTTAAGTGGASGGAAAAVCACT-3′). Specific fragments about 1.7 kb amplified from all eight symptomatic samples using the potyvirus-specific primers were sequenced and analyzed by BLASTn search in GenBank. The results showed that all the sequences amplified shared 83% to 88% nucleotide identities with sequences of Hippeastrum mosaic virus (HiMV) previously deposited in GenBank containing the partial nuclear inclusion body (NIb) gene, full-length coat protein (CP) gene, and 3′-untranslated region. The CP gene of the HiMV Ji’nan isolate from China was deposited in GenBank (KX462993); it shared 89% nucleotide sequence identity and 96% amino acid sequence identity with the corresponding gene of Amaryllis potyvirus (AY566239) from Taiwan, which is a strain of HiMV (Wylie and Jones 2012). In addition, one nonsymptomatic and all eight symptomatic samples were tested with plate-trapped antigen (PTA) indirect ELISA, using an antibody specific for the potyvirus group (Agdia, Elkhart, IN). All the symptomatic samples produced positive reactions (the absorbance values ranged from 1.131 to 1.472), whereas the nonsymptomatic plant showed a negative reaction (the absorbance value was 0.322). To further confirm the presence of a potyvirus, electron microscopic examinations of negatively stained samples of randomly selected leaf-dip preparations from the symptomatic plants revealed flexuous filamentous viral particles of 700 to 800 × 13 nm, resembling previously described HiMV (Alexandre et al. 2011). Chenopodium quinoa and healthy Hippeastrum plants were inoculated mechanically using the extracts of symptomatic Hippeastrum. Necrotic local lesions were appeared in the C. quinoa at 7 days post inoculation (dpi) and mosaic symptoms appeared in the Hippeastrum plants at 30 dpi. The tests of leaves with local necrotic lesions in C. quinoa and mosaic leaves in the inoculated Hippeastrum plants showed positive reactions by RT-PCR using the primers special to HiMV CP (HiMV CP-F: 5′-CTGAATCGTATATCGTGATGC-3′ and HiMV CP-R: 5′-TGTCCCGACCTTGCCATC-3′). To our knowledge, this is the first report of HiMV in mainland China. To reduce the potential environmental risks of this virus, a quick and sensitive detection technique should be developed for the flower trade in China.References:Alexandre, M. A. V., et al. 2011. J. Plant Pathol. 93:643. ISI, Google ScholarChen, J., et al. 2001. Arch. Virol. 146:757. https://doi.org/10.1007/s007050170144 Crossref, ISI, Google ScholarJordan, R., and Hammond, J. 1991. J. Gen. Virol. 72:25. https://doi.org/10.1099/0022-1317-72-1-25 Crossref, ISI, Google Scholarvan der Vlugt, R. A. A., and Berendsen, M. 2002. Eur. J. Plant Pathol. 108:367. https://doi.org/10.1023/A:1015644409484 Crossref, ISI, Google ScholarWylie, S. J., and Jones, M. G. K. 2012. Arch. Virol. 157:1471. https://doi.org/10.1007/s00705-012-1319-6 Google ScholarDetailsFiguresLiterature CitedRelated Vol. 101, No. 6 June 2017SubscribeISSN:0191-2917e-ISSN:1943-7692 Metrics Article History Issue Date: 17 May 2017Published: 4 Apr 2017First Look: 14 Feb 2017Accepted: 8 Feb 2017 Pages: 1064-1064 Information© 2017 The American Phytopathological SocietyCited byHippeastrum mosaic virusCABI Compendium, Vol. CABI CompendiumNovel viruses associated with plants of the family Amaryllidaceae in South Africa19 July 2021 | Archives of Virology, Vol. 166, No. 10Hippeastrum spp. (Amaryllis)6 June 2020Revealing the complex genetic structure of cultivated amaryllis (Hippeastrum hybridum) using transcriptome-derived microsatellite markers13 July 2018 | Scientific Reports, Vol. 8, No. 1