壳聚糖
氯化铵
聚乙二醇
核化学
纳米颗粒
牛血清白蛋白
PEG比率
钠
毒品携带者
材料科学
化学
高分子化学
药物输送
有机化学
色谱法
纳米技术
经济
财务
作者
Xu Yang,Yumin Du,Ronghua Huang,Gao Le-ping
出处
期刊:Biomaterials
[Elsevier]
日期:2003-12-01
卷期号:24 (27): 5015-5022
被引量:198
标识
DOI:10.1016/s0142-9612(03)00408-3
摘要
N-(2-hydroxyl) propyl-3-trimethyl ammonium chitosan chloride (HTCC) is water-soluble derivative of chitosan (CS), synthesized by the reaction between glycidyl-trimethyl-ammonium chloride and CS. HTCC nanoparticles have been formed based on ionic gelation process of HTCC and sodium tripolyphosphate (TPP). Bovine serum albumin (BSA), as a model protein drug, was incorporated into the HTCC nanoparticles. HTCC nanoparticles were 110-180 nm in size, and their encapsulation efficiency was up to 90%. In vitro release studies showed a burst effect and a slow and continuous release followed. Encapsulation efficiency was obviously increased with increase of initial BSA concentration. Increasing TPP concentration from 0.5 to 0.7 mg/ml promoted encapsulation efficiency from 46.7% to 90%, and delayed release. As for modified HTCC nanoparticles, adding polyethylene glycol (PEG) or sodium alginate obviously decreased the burst effect of BSA from 42% to 18%. Encapsulation efficiency was significantly reduced from 47.6% to 2% with increase of PEG from 1.0 to 20.0 mg/ml. Encapsulation efficiency was increased from 14.5% to 25.4% with increase of alginate from 0.3 to 1.0 mg/ml.
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