Levels of interleukin‐37 in gingival crevicular fluid, saliva, or plasma in periodontal disease

BACKGROUND AND OBJECTIVE: The aim of this study was to compare the levels of levels of interleukin-37 (IL-37) in gingival crevicular fluid, saliva and plasma in patients with periodontal disease and patients with healthy periodontium and to correlate these levels with clinical parameters. MATERIAL AND METHODS: Samples of gingival crevicular fluid, whole saliva and plasma were collected from systemically healthy, nonsmoker periodontally healthy controls (group 1, n = 20), gingivitis patients (group 2, n = 20) and chronic periodontitis patients (group 3, n = 20). Full-mouth clinical periodontal parameters, including probing depth, plaque index, gingival index and bleeding on probing, were also recorded. IL-37 levels in the biofluid samples were determined by ELISA. Data were tested statistically using the Kruskal-Wallis test followed by the Mann-Whitney U-test. RESULTS: The concentration of IL-37 in gingival crevicular fluid was significantly lower in group 3 than in groups 1 and 2 (p = 0.001), whereas the total amounts in gingival crevicular fluid samples were similar (p > 0.05). The salivary and plasma concentrations of IL-37 were similar in the study groups (p > 0.05). There were negative correlations between gingival crevicular fluid IL-37 concentrations and gingival crevicular fluid volume in all groups (p < 0.05). There was also a negative correlation between the gingival crevicular fluid IL-37 concentration and gingival index in group 3 (p < 0.05). CONCLUSIONS: IL-37 was expressed in all biofluids. According to our findings, the total amount of IL-37 in gingival crevicular fluid, or salivary or plasma concentrations of IL-37, may not be useful diagnostic markers to differentiate periodontal disease and the periodontally healthy condition. The difference in gingival crevicular fluid IL-37 concentration between the study groups may be a result of the variation in gingival crevicular fluid volume, as suggested by the negative correlation between gingival crevicular fluid volume and gingival crevicular fluid IL-37 concentration. In the light of our findings, it seems that IL-37 is not involved in periodontal disease. Further comprehensive studies may clarify this issue more clearly.