Fluorescent DNA Biosensors on Silica Microspheres

A novel DNA biosensor system on silica microspheres as solid carriers which based on the fluorescence resonance energy transfer (FRET) was presented in this work when CdTe quantum dots (QDs) were as energy donors and Au nanoparticles (AuNPs) were as energy accepters. Compared with the fluorescent intensity of CdTe QDs, the fluorescent intensity of DNA biosensors decreased extremely, which indicated that the FRET occurred between CdTe QDs and AuNPs. The biosensor system would have a certain degree recovery of fluorescence when the complementary single stranded DNA was introduced into this system. The DNA detection results indicated that this novel fluorescent DNA probe system could recognize the existence of complementary target DNA or not.