Histidine Production by Corynebacterium glutamicum Mutants, Multiresistant to Analogs of Histidine, Tryptophan, Purine and Pyrimidine

Abstract The histidine productivity of 1,2,4-triazole-3-alanine (TRA)-resistant histidine producer, Corynebacterium glutamicum KY–10260 was improved by successive additions of such markers as purine, pyrimidine, histidine and tryptophan analog-resistance to the mutant. A selected mutant AT–83, multi-resistant to 6-mercaptoguanine, 8-azaguanine, 4-thiouracil, 6-methylpurine, TRA and 5-methyltryptophan, accumulated twice as much histidine as KY–10260 in the culture medium. The level of histidine production by this mutant reached 15 mg/ml or 10% (w/w) of the initial suagr in the medium containing 6% (as glucose concentration) cane molasses and 9% sucrose as carbon sources. 2-Fluoroadenine-resistant mutants produced adenine in addition to histidine.