Design and Simulation of Micropillar based Microfluidic platform for the growth of Organoids in-line with Drug Testing

Models derived from the latter, such as spheroids or organoids are more similar to the in-vivo environment and thus should be considered for drug testing. When entwined with a microfluidic platform imply powerful assay advantages like control media flow, minute cell, and reagents requisition during the culture. In this work, the novel approach is to cultivate healthy and diseased organoids in the same microfluidic platform through a controlled microvalve. Design and simulation are performed using AUTOCAD, CLEWIN, and COMSOL. The novel micropillar microfluidic device was fabricated using photolithography followed by soft lithography. The fabricated device is temporarily bonded to a glass slide through the double tape to check the fluid flow. The phenomenon is visualized by subjecting a solution of methylene blue across the device.