Single‐Cell Antimicrobial Susceptibility Testing via Stimulus‐Responsive Resonance Raman Scattering Imaging

using the SRRRS-imaging technique, which is 3-4 orders of magnitude lower than conventional optical probes. We further constructed a SRRRS-imaging-derived antibiotic breakpoints library for Pseudomonas aeruginosa (P. aeruginosa, PAK), enabling rapid susceptibility testing for 14 antibiotics within 3 h without bacterial culture. Clinical validation with 30 intensive care unit patients demonstrated 93% accuracy, offering a transformative tool for guiding timely antibiotic prescriptions and improving outcomes in PAK-related infections.