The catalytic capability of 67 Strains of mold,yeast and bacteria,for the biosynthesis of arbutin,were investigated.The molecular weight and configuration of arbutin were determined by HPLC,Electron Ionization-Mass Spectrometry and polarimetry.The results indicated that there were more than 70 % of microorganisms which could produce enzyme for catalyzing the biosynthesis of arbutin and the superior product were β-arbutin.