Immunophenotyping of 175 Case with Acute Leukemia by Flow Cytometry

Obiective To investigate the significance of immunophenotype in acute leukemia via flow cytometry,and study the relationship of immunophenotype and FAB phenotype.Methods Immunophenotypes were detected by three-color flow cytometry(FCM)and CD45/SSC gating in 175 cases of acute leukemia(AL)and antigentic expressions were analysed.Results FAB classification and immunophenotype consistent rate of 90.28%(158/175),175 cases of acute leukemia detected in B-ALL44 cases,accounting for 25.14%,antigen positive rate of its main expression in the order of CD_(19),CD_(22),CD_(79)a;T-ALL19 cases,accounting for 10.86%,the positive rate of antigen expression and its main descending order of HLA-DR,CD_(34),CD_5,CD_7,cCD_3,CD_8,CD_(38);non M3 AML103 cases,accounting for 58.86%,its the positive rate of antigen expression in the order of major CD_(33),CD_(117),CD_(38),CD_(13),HLA-DR,MPO,CD_(34),CD_(123),CD_(15),CD_(64);were found in AML-M3 6 patients,accounting for 0.34%,and its main antigen expression of MPO,CD_(13),CD_(33),CD_(117),CD_(123),CD_(64).Conclusion Immunophenotype Plays an important role in the classification of acute leukemia and is a supplementary and correction of FAB phenotype.It can enhance the exactness of diagnosis and needs to be standardized.