Alkaloid from Sophora flavescens Ait was extracted with three methods: ultrasonic method, circumfluence of condensation,and dipping in normal temperature. The extractives are mostly matrine and oxymatrine.The effect of extractives on lustrating Trichodinella of fish was also studied.The results showed that the alkaloid from Sophora flavescens Ait could lustrate Trichodinella ninuta efficiently. With the different extractives through different extracting methods,the effect on lustrating Trichordinella nenuta of fish were also different. The content of matrine was highest in extractive by ultrasonic method among three methods and the effect on lustrating Trichodinella of fish was also the best,which meant that the matter lustrating Trichodinella is matrine.