Liquid fermentation of Phellinus igniarius for production of polysaccharide

Objective To investigate the effects of different fermentation conditions on liquid fermentation of Phellinus igniarius for production of polysaccharide.Methods The main fermentation parameters,kinds of media,initial pH values,fermentation temperature,and rotation speed,were optimized by using orthogonal test.Ampliation tests of fermentation were done in a 5 L fermenter.Results The optimal fermentation parameters were potato dextrose(PD) medium with 0.3 g/L vitamin B1 and 0.3 g/L vitamin B2,initial pH value 5,temperature 28 ℃,and rotation speed 180 r/min.Under the optimal fermentation conditions,the maximal intracellular polysaccharide(IPS) and exopolysaccharide(EPS) production was 5.80 and 2.37 g/L in shake flasks,while the maximal IPS and EPS production reached 5.85 and 2.30 g/L in 5 L fermenter,respectively.Conclusion This study provides reference for industrialization of P.igniarius liquid fermentation.