The present article investigated the application of acid protease in alcohol fermentation.The results of the experiment showed that the fermentation period might be decreased and the alcohol rate of raw materials might be increased when suitable acid protease was added in alcohol fermentation.The optimal technology conditions were got which the additive amount of acid protease was 14 u/g,the pH was 4~6,the main temperature of fermentation was 35~38℃,the fermentation time was 48~60h.With these conditions,the alcohol rate of raw materials was increased by 4.34% as compared with no adding acid protease.