Production of(R)-Mandelic Acid by Asymmetric Degradation of the Racemate with Brevibacterium sp.CCSYU10011

(R)-mandelic acid is a useful chiral synthon for the production of various pharmaceutical.In order to prepare(R)-mandelic acid from rac-mandelic acid,Brevibacterium sp.CCSYU10011 was screened from various microorganisms,of which the resting cells could convert the racmate to(R)-isomer.The process of bioconversion showed that the microorganism asymmetricly degraded(S)-mandelic acid from the racemate and(R)-mandelic acid was maintained.The factors affecting biotransformation efficiency were investigated,including temperature,pH,substrate concentration and cell biomass.Under the optimal conditions,48.7% yield and 99% enantiomeric excess value were obtained.