Objective:To study the effect of different rhizome grades on the yield of Paris polyphylla var.yunnanensis.Methods:To compare survival percentage,increased times of rhizomes size,weigh and yield of different rhizome grades of Paris polyphylla var.yunnanensis cultivated for 3 years.Results: the most suitable rhizome grade was selected out.Conclusion:the most suitable rhizome grade is 10g and increased times of rhizomes weigh can reach 3 times.