Comparison of rutin and syringin content between tissue culturing seedlings and botanical drug of Saussurea involucrata

To compare the rutin and syringin content in tissue culturing seedlings and in botanical drug of Saussurea involucrata.The HPLC with Hydro-RP C18 (4.6 mm x 250 mm, 5 microm) column was used, a mixture of acetonitrile-water (5:95) was used as a mobile phase, with flow rate of 1 mL x min(-1), column temperature at 25 degrees C and detection wavelength at 220 nm.The effective constituents of tissue culturing seedlings were almost similar to the botanical drug. And syringin in tissue culturing seedlings was increased 4.35 times.It has a good prospect to acquire high-quality S. involucrata by tissue culturing seedlings.