Extracted by 45% ethanol,purified by polycaprolactam and the rutin was used as a standard compound,the total flavonoid content of Gynura divaricata (L.) DC.was quantitated by measurement of absorbance at 510 nm.The results show that the content of the whole plant is 1.43%,the leaves 1.16%,the stem 2.60%,the root 4.28%.This study will provide scientific basis on producing medicinal components of flavonoids from Gynura divaricata(L.) DC..