Correlation and role of TRAIL receptor DR5 and Ki67 expressed on primary acute leukemia cells

Objective To explore the role and the relationship between tumor necrosis factor-related apoptosis-inducing ligand(TRAIL) receptor DR5 and Ki67 on acute leukemia cells.Methods Bone marrow mononuclear cells(BMMNC) from 20 cases of acute leukemia(acute leukemia group) and 10 cases of non-malignant hematological diseases(control group) were cultured for 72 hours,the expression of DR5 was analyzed by flow cytometry,and the expression of Ki67 by immunocytochemical staining.Results The expression of Ki67 on BMMNC from acute leukemia cells was significantly higher than that of control group 32.3±8.2 vs 15.4±3.9(P0.05).The expression of TRAIL-DR5 was low and there was no significant difference as compared with that of control group,12.2±4.3 vs 9.9±2.3(P0.05).The expression of Ki67 was negatively correlated with that of TRAIL-DR5(r=-0.459,P0.05).Conclusion The expression and the relationship of TRAIL receptor DR5 and Ki67 on acute leukemia cells suggest the abnormality in the apoptosis or proliferation of leukemia cells.The simultaneous assays of the expression of DR5 and Ki67 on acute leukemia cells have a better predictive value in the pathogenesis of acute leukemia than checking singularly.