Molecular marker of disease resistance gene is stable accurate and efficient. It's application in marker-assisted selection can accelerate the conventional breeding process and raise its efficiency through the direct selection on genotype but not phenotype. Outline of development of marker-assisted selection in plant breeding for disease resistance was reviewed in this paper. Technology of various DNA molecular tagging and selection method of molecular marker linked to crop disease resistance gene was introduced. Current status in the area of molecular tagging on disease resistance genes in sugarcane is also enclosed. At the same time prospects of molecular marker-assisted selection in sugarcane breeding for disease resistance were forecasted.