Proteomics Analysis of Purified Murine Renal Lipid Droplets

Abstract Lipid droplets (LDs) play pivotal roles in lipid metabolism and cellular signaling. However, the involvement of LDs in kidney function remains incompletely elucidated. In this study, we employed immunodetection and high-performance liquid chromatography (HPLC) coupled with mass spectrometry to comprehensively profile the proteomics of LDs in the mouse kidney. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to delineate the potential function of the identified proteins. In addition, Protein-protein interaction (PPI) network was applied to identify cluster modules within the protein interaction network. A total of 134 proteins were identified, which reveals an intricate network of proteins associated with lipid metabolism, transport, and cellular homeostasis. GO and KEGG pathway enrichment analyses highlighted the involvement of these proteins in key biological processes, including lipid metabolic process, and regulation of LDs. PPI network analysis further uncovered a network comprising 112 nodes and 594 edges. These findings offer novel insights into the functional role of LDs in renal physiology.