Real-Time, Ultrasensitive, and High-Throughput Monitoring of Adeno-Associated Virus Production via an Aldehyde-Functionalized Dual-Capture MetaSPR Fishing Sensor

GC/mL, representing a 250-fold sensitivity improvement over current label-free techniques. The biosensor surface can be regenerated more than 42 cycles without performance loss, which significantly reduces the per-test costs. This innovation resolves a critical bottleneck in gene-therapy manufacturing by integrating high-throughput screening, real-time process analytics, and one-step operation.