代谢组
骨骼肌
代谢物
内科学
分解代谢
肌萎缩
内分泌学
医学
支链氨基酸
生物
生理学
新陈代谢
氨基酸
生物化学
亮氨酸
作者
Parastoo Fazelzadeh,Roland W. J. Hangelbroek,Michael Tieland,Lisette C. P. G. M. de Groot,Lex B. Verdijk,Luc J. C. van Loon,Age K. Smilde,Rodrigo D. A. M. Alves,Jacques Vervoort,Michael Müller,John van Duynhoven,Mark V. Boekschoten
标识
DOI:10.1021/acs.jproteome.5b00840
摘要
Populations around the world are aging rapidly. Age-related loss of physiological functions negatively affects quality of life. A major contributor to the frailty syndrome of aging is loss of skeletal muscle. In this study we assessed the skeletal muscle biopsy metabolome of healthy young, healthy older and frail older subjects to determine the effect of age and frailty on the metabolic signature of skeletal muscle tissue. In addition, the effects of prolonged whole-body resistance-type exercise training on the muscle metabolome of older subjects were examined. The baseline metabolome was measured in muscle biopsies collected from 30 young, 66 healthy older subjects, and 43 frail older subjects. Follow-up samples from frail older (24 samples) and healthy older subjects (38 samples) were collected after 6 months of prolonged resistance-type exercise training. Young subjects were included as a reference group. Primary differences in skeletal muscle metabolite levels between young and healthy older subjects were related to mitochondrial function, muscle fiber type, and tissue turnover. Similar differences were observed when comparing frail older subjects with healthy older subjects at baseline. Prolonged resistance-type exercise training resulted in an adaptive response of amino acid metabolism, especially reflected in branched chain amino acids and genes related to tissue remodeling. The effect of exercise training on branched-chain amino acid-derived acylcarnitines in older subjects points to a downward shift in branched-chain amino acid catabolism upon training. We observed only modest correlations between muscle and plasma metabolite levels, which pleads against the use of plasma metabolites as a direct read-out of muscle metabolism and stresses the need for direct assessment of metabolites in muscle tissue biopsies.
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