微血管
生物物理学
纳米颗粒
血脑屏障
聚山梨酯
药物输送到大脑
化学
共焦显微镜
中枢神经系统
生物化学
细胞生物学
材料科学
生物
纳米技术
血管生成
内分泌学
癌症研究
肺表面活性物质
作者
Peter Ramge,Ronald E. Unger,Jens B. Oltrogge,Dietmar Zenker,David J. Begley,Jörg Kreuter,Hagen von Briesen
标识
DOI:10.1046/j.1460-9568.2000.00078.x
摘要
Abstract Certain drugs such as dalargin, loperamide or tubocurarine are not transported across the blood–brain barrier (BBB) and therefore exhibit no effects on the central nervous system. However, effects on the central nervous system can be observed when these drugs are loaded onto polybutylcyanoacrylate (PBCA)‐nanoparticles and coated with polysorbate 80. The mechanism by which these complexed nanoparticles cross the BBB and exhibit their effects has not been elucidated. Cultured microvessel brain endothelial cells of human and bovine origin were used as an in vitro model for the BBB to gain further insight into the mechanism of uptake of nanoparticles. With cells from these species we were able to show that polysorbate 80‐coated nanoparticles were taken up by brain endothelial cells much more rapidly and in significantly higher amounts (20‐fold) than uncoated nanoparticles. The process of uptake was followed by fluorescence and confocal laser scanning microscopy. The results demonstrate that the nanoparticles are taken up by cells and that this uptake occurs via an endocytotic mechanism.
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