ENZYMATIC ACTIVITY OF THE FUSION PROTEIN OF TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE WITH DNA-BINDING PROTEIN SSO7D FROM S. SOLFATARICUS

Gene fusion techniques have demonstrated promising results in developing more active, processive DNA polymerases. In the present work, we isolated and investigated the activity of a fusion enzyme combining terminal deoxynucleotidyl transferase (TdT) with the DNA-binding protein from Sulfolobus solfataricus (Sso7d). The fusion proteins exhibit enhanced 3′-5′ exonuclease activity, which substantially impairs their nucleotide incorporation efficiency.