毕赤酵母
木聚糖酶
化学
水解物
食品科学
水解
酵母
香菇属
小黑麦
响应面法
生物化学
色谱法
植物
重组DNA
酶
生物
蘑菇
基因
作者
Chanjuan Liu,Wenjing Zhang,Yanjiao Li,Ke Pan,Kehui Ouyang,Xiaozhen Song,Xiaowen Xiong,Yitian Zang,Lei Wang,Mingren Qu,Xianghui Zhao
标识
DOI:10.1016/j.ijbiomac.2021.12.178
摘要
The current study displayed a xylanase from Lentinula edodes on the surface of Pichia pastoris (sdLeXyn) and investigated its properties and effects on the wheat hydrolysis. Fluorescence microscope results showed that sdLeXyn was successfully anchored and displayed on the surface of P. pastoris X-33 cells. The highest activity of sdLeXyn was obtained at pH 3.0 and 50 °C. The sdLeXyn exhibited anti-high temperature property and showed broad temperature adaptability (>55% of the highest activity at 20-80 °C). The sdLeXyn was very stable at room temperature and could remain functionally stable at 50 °C for 3 h. The Km value was greater in sdLeXyn than that in free recombinant L. edodes xylanase. The sdLeXyn exhibited well resistance to Mn2+, Zn2+, Ca2+, Na+, Cu2+, Mg2+, K+, Ni2+ (1 mM and 5 mM) except Cu2+, which reduced the sdLeXyn activity by 54.5% at 5 mM dosage. The activity of sdLeXyn was increased by 42.6% by 5 mM Mn2+, 5 mM DTT, Trition X-100, and Tween 20 did not affect the activity of sdLeXyn, but SDS and EDTA slightly reduced it by 12.8% and 14.6%, respectively. The sdLeXyn could resist the degradation of pepsin, efficiently hydrolyzed wheat and reduced the viscosity of wheat hydrolysate. Current data indicate that the sdLeXyn has a potential as a feed additive to improve the utilization of wheat in poultry production.
科研通智能强力驱动
Strongly Powered by AbleSci AI