De Novo Assembly of Linked Reads Using Supernova 2.0

The recent emergence of “third-generation” sequencing platforms which address shortcomings of standard short reads has allowed the resolution of complex genomic regions during genome assembly. However, sequencing costs for third-generation platforms continue to be high. Novel approaches that leverage the low cost of short-read sequencing while capturing long-range information have been developed. In this chapter, we focus on one such approach, the 10x Genomics’ Chromium system. We demonstrate the assembly of the B73 maize reference genome using the Supernova assembler. We also offer suggestions on how one might improve the resulting assembly through analysis of assembly metrics.