反硝化细菌
反硝化
生物膜
化学
硝化作用
环境化学
胞外聚合物
蛋白质细菌
磷
水力停留时间
移动床生物膜反应器
氮气
酸杆菌
序批式反应器
强化生物除磷
流出物
环境工程
生物
生物化学
废水
活性污泥
有机化学
细菌
环境科学
基因
遗传学
16S核糖体RNA
作者
Dandan Pan,Sicheng Shao,Jinfeng Zhong,Minghui Wang,Xiangwei Wu
标识
DOI:10.1016/j.biortech.2022.126726
摘要
The long-term moving bed biofilm reactor (MBBR) with carrier-attached biofilm was successfully operated for simultaneous removal of nitrogen, phosphorus, and COD at various C/N ratios. Results indicated that 99.60%, 63.58%, 78.94%, and 59.64% of NH4+-N, NO3--N, TN, and TP were removed at C/N ratio, hydraulic retention time (HRT), and carrier film amount of 5, 40 h, and 1.2 mg·g-1. Nitrogen balance analysis showed that more than 89% of nitrogen (C/N = 20, 15, 10, 5) was converted to gas products. Extracellular polymeric substances (EPS), electron transport system activity (ETSA), and enzyme activity of biofilm were evaluated. Protein (PN)/polysaccharose (PS) values and ETSA decreased with the decrease of C/N ratios. Metagenomics sequencing further revealed that the prominent phyla for nitrogen and phosphorus removal were identified including Proteobacteria, Acidobacteria, Nitrospirae, and Chloroflexi. Proteobacteriaand Gammaproteobacteria were identified as the dominant denitrifying phosphate accumulating organisms (PAO) at the phylum and class level, respectively.
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