作者
Liming Zhang,Daxi Wang,Pei Yong Shi,J. Li,Jichen Niu,Jielong Chen,Gang Wang,Linjuan Wu,Lu Chen,Zi Jiang Yang,Shifang Li,Jianghong Meng,Fengyu Ruan,Yue He,H.-X. Zhao,Zirui Ren,Yibaina Wang,Lei Yang,Xiaolu Shi,Yudong Wang,Qiyong Liu,Junhua Li,Penghua Wang,Jinglin Wang,Yibin Zhu,Gong Cheng
摘要
The commensal microbiota of the mosquito gut plays a complex role in determining the vector competence for arboviruses. In this study, we identified a bacterium from the gut of field Aedes albopictus mosquitoes named Rosenbergiella sp. YN46 ( Rosenbergiella_ YN46) that rendered mosquitoes refractory to infection with dengue and Zika viruses. Inoculation of 1.6 × 10 3 colony forming units (CFUs) of Rosenbergiella_ YN46 into A. albopictus mosquitoes effectively prevents viral infection. Mechanistically, this bacterium secretes glucose dehydrogenase ( Ry GDH), which acidifies the gut lumen of fed mosquitoes, causing irreversible conformational changes in the flavivirus envelope protein that prevent viral entry into cells. In semifield conditions, Rosenbergiella_ YN46 exhibits effective transstadial transmission in field mosquitoes, which blocks transmission of dengue virus by newly emerged adult mosquitoes. The prevalence of Rosenbergiella_ YN46 is greater in mosquitoes from low-dengue areas (52.9 to ~91.7%) than in those from dengue-endemic regions (0 to ~6.7%). Rosenbergiella_ YN46 may offer an effective and safe lead for flavivirus biocontrol.