微流控
球体
类有机物
纳米技术
化学
材料科学
生物
细胞生物学
生物化学
体外
作者
Yihe Wang,Mengqi Liu,Yu Zhang,Hong Liu,Lin Han
出处
期刊:Lab on a Chip
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:23 (5): 1080-1096
被引量:9
摘要
Droplet microfluidic techniques have long been known as a high-throughput approach for cell manipulation. The capacity to compartmentalize cells into picolitre droplets in microfluidic devices has opened up a range of new ways to extract information from cells. Spheroids and organoids are crucial in vitro three-dimensional cell culture models that physiologically mimic natural tissues and organs. With the aid of developments in cell biology and materials science, droplet microfluidics has been applied to construct spheroids and organoids in numerous formats. In this article, we divide droplet microfluidic approaches for managing spheroids and organoids into three categories based on the droplet module format: liquid droplet, microparticle, and microcapsule. We discuss current advances in the use of droplet microfluidics for the generation of tumour spheroids, stem cell spheroids, and organoids, as well as the downstream applications of these methods in high-throughput screening and tissue engineering.
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