Analysis of Flavonoids of Flower of Robiniapseu Doacacia L. by HPLC-DAD-ESI-MS/MS

Objective To analyze the flavonoids of the flower of Robiniapseu doacacia L.by HPLC-DAD-ESI-MS/ MS(DAD:diode array detection).Methods An Agilent Zorbax SB-Aq column(250 mm × 4.6 mm,5 μm) was used to separate those flavonoids in the flower.The mobile phase consisting of water containing 1.0 % acetic acid and acetonitrile was used for gradient elution.The flow rate was 1.0 mL /min.ESI-MS was applied for qualitative analysis under positive ion mode.Results Under LC/MS condition,11 flavonoid glycosides in the flower of Robiniapseu doacacia L.were identified by mass spectrometry and structure-relevant fragment ions.Conclusion The method of HPLC-DAD-ESI-MS/MS can be simple and reliable to identify the chemical constituents of the flower of Robiniapseu doacacia L.